lesson 9 introduction to biochemistry

SCI260 – Introduction to Biochemistry

Lesson #9

(1) Which of the double-stranded DNA sequences below will require more heat to

separate the two strands (select Option A or Option B)? (2) Why is more heat is required

to separate the strands?

Option A

5’-GGACTCCCGGGTGAG-3’

3’-CCTGAGGGCCCACTC-5’

Option B

5’-AACTGTTTAAAGACA-3’

3’-TTGACAAATTTCTGT-5’

(3) What will be the mRNA sequence that is produced during the transcription of the

DNA sequence below? Hint: As we discussed in class, there is a slight difference in the

nucleotides used in DNA vs RNA.

5’-ACGTCAGGTTTCCGT-3’

(4) Based on what was discussed in Lecture 9, name four (4) enzymes/proteins that play

a role in human DNA replication, and briefly describe the specific role each plays during

human DNA replication.

You may use the internet and/or your textbook to assist you. Each answer is worth 25

points (be sure to answer ALL four questions) giving you a maximum total of 100 points

for this assignment. Please DO NOT copy your answers directly from the textbook or the

internet.