You are working in a lab and come across a sample of blood that has the Flu virus in it. You want to grow it out and see it replicate. You inoculate the blood onto Trypticase Soy Agar (general purpose). You put it in the incubator for the required growth time and come back and check on it. There’s nothing on the plate. Why?
YOU ARE ATTEMPTING TO PURIFY A PROTEIN TO “CRYSTALLOGRAPHIC QUALITY” (I.E. VERY PURE). THIS PROTEIN HAS A 6X HIS TAG, A PI OF 8.6 AND IS ABOUT 20 KDA. WHAT STEPS WOULD YOU TAKE TO PURIFY YOUR PROTEIN AND TO ASSESS ITS PURITY?
You are working in a lab and come across a sample of blood that has the Flu virus in it. You want to grow it out and see it replicate. You inoculate the blood onto Trypticase Soy Agar (general purpose). You put it in the incubator for the required growth time and come back and check on it. There’s nothing on the plate. Why?
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The post YOU ARE ATTEMPTING TO PURIFY A PROTEIN TO “CRYSTALLOGRAPHIC QUALITY” (I.E. VERY PURE). THIS PROTEIN HAS A 6X HIS TAG, A PI OF 8.6 AND IS ABOUT 20 KDA. WHAT STEPS WOULD YOU TAKE TO PURIFY YOUR PROTEIN AND TO ASSESS ITS PURITY? appeared first on Cheap Nursing Tutors.
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